Cerebral organoids recapitulate interneuron chain migration in the human cortex

Unser Preprint ist online!

Source: https://www.biorxiv.org/content/10.64898/2026.09.30.729488v1

Das hat Sakurako dazu auf Linkedin zu sagen:

🧠 I’m very excited to share our new preprint from the Knoblich Lab at Institute of Molecular Biotechnology, now out on bioRxiv! 🧫 Can human brain organoids recapitulate developmental processes that extend beyond birth? We found that long-term cerebral organoids generate late-born CGE interneurons that organise into striking migratory streams, resembling a developmental process that continues into postnatal life in the human brain. By combining live imaging, 2D explant assays, mathematical modelling, spatial and single-cell transcriptomics, we were able to follow these streams and begin to dissect the mechanisms underlying their formation. We find that late-born interneurons acquire an intrinsic propensity for chain migration, while interactions with astrocytes contribute to their organisation into streams. Remarkably, these cells also show strong transcriptional correspondence to the recently described human postnatal entorhinal cortex stream. Finally, we identified a stream-associated gene network enriched for genes linked to neurodevelopmental disorders, including ASD and epilepsy, and found that perturbing CNTN5 and FGF14 disrupts distinct aspects of stream migration. This work has been a huge part of my PhD, and I’m so incredibly happy to finally share it! 💖💖 A massive thank you to everyone who contributed to this project: Mark Alan Noble, Ph.D., Michael Fischer, Léo Meyer, Maria Novatchkova, Ema Nováková, Denise Van Der Heijden, Lucas Barea Moya, Susana Gonzalez Granero, Jose Manuel Garcia-Verdugo, Sara Merino Aceituno, Nina Corsini, and Juergen Knoblich!